> Skip to contents (site navigation)
The main aims of the project Prague Infrastructure for Structural Biology and Metabolomics II are the following:
Supported by Ministry of Education, Youth and Sports of the Czech Republic, grant LO1509, 2015-2020
Cooperation with 1st Medicinal Faculty, Institute of Chemical Technology Prague and General University Hospital in Prague
Obesity, diabetes mellitus type 2 (T2DM) and their complications are one of the most pressing medical problems today. One of the central processes of bonding obesity, T2DM and their complications is considered subclinical inflammation characterized by infiltration of adipose tissue by immunocompetent cells, especially macrophages. In addition to macrophages in the development of subclinical inflammation in adipose tissue is likely to cooperate also other immunocompetent cells. The project aims to clarify the role of immunocompetent cells in adipose tissue and their interaction with circulating cells in etiopathogenesis of imunocompetent subclinical inflammation, insulin resistance and other associated complications. The cellular composition of adipose tissue in terms of representation of immunocompetent cells and their relationship to their precursors in peripheral blood of patients with obesity and obesity and/or diabetes mellitus conditions and after weight reduction (low calorie diet, weight loss surgery) will be monitored. The project will contribute to improving the understanding of basic etiopathogenic mechanisms leading to the emergence of T2DM and associated complications. It may also help to identify new therapeutic targets, allowing to better meet the current global pandemic of obesity, T2DM and associated diseases.
Supported by Ministry of Health, grant NT/13299 - 4
(Miroslav Kolařík, Vladimír Havlíček)
The capacity to enter hibernation and/or daily torpor is an adaptive physiological trait that enables survival of climatic extremes and food scarcity. Here we plan to investigate variation of organismal and cellular responses to multiple stressors in hibernating bats in relation to torpor-arousal patterns and the associated profound changes of body temperature and metabolism. We will quantify parameters of health and the microbiome of bats, identify pathogenic microorganisms and their factors of virulence and link pathogenic pressure and the cellular response to stressors with genomic variability. Using cutting-edge in vitro tools of tissue-derived bat cells, we will examine
Supported by Czech Science Foundation, grant No. 17-20286S (2017-2019)
The project is focused on the development of a technology for biodegradation of persistent pharmacologically active ingredients (APIs: diclofenac, ibuprofen, ketoprofen and sulfamethoxazole, called “emerging pollutants”) which are continuously introduced in aquatic environment through their high consumption by society. Technology is based on the application of natural bacterial isolates increasing the efficiency of the APIs biodegradation in wastewater in the course its treatment in wastewater treatment plant (WWTP). The goal of the project is to characterize existing bacterial strain capable of degradation of selected APIs, screening and testing of alternative microorganisms, characterization of the degradation processes in water and activated sludge mediated by microorganisms, monitoring of a pollutant during the treatment process and development of analytical procedures for the determination of different forms of the APIs. Newly developed technology will be tested on a pilot scale model of the WWTP. Its implementation will significantly reduce pollution of surface waters and increase the sustainability of water resources.
Supported by the Technology Agency of the Czech Republic, EPSILON Programme, grant TH02030337 (2017-2020)
Cooperation with Medical Faculty, Palacký University in Olomouc, Dr. Milos Petrik
Mixed infections represent an analytical challenge for any microbiology laboratory. In this project high mass resolution spectrometry will be used in newly developed rat experimental models as a molecular tool sensitive enough to describe and monitor mutual microbial combat in a rat model. An early disease detection and whenever possible, non-invasive analytical tools will be developed based on microbial siderophores, specific disease biomarkers, to be detected in urine of infected animals. Special attention will be paid to mixed infections in rat lungs caused by Aspergillus and Pseudomonas, common pathogens participating in cystic fibrosis or patients with chronic obstructive pulmonary disease. The biofilm formation and its decay will be monitored by light and electron microscopy. Multimodal imaging approach, supported by software development, will rely on in vivo data obtained by positron emission tomography/computed tomography. The efficiency of antibacterial/antifungal treatment will be monitored by silencing of siderophore production.
Supported by Czech Science Foundation, grant No. 19-10907S (2019-2021)
^ Page top