Chromatogram

Chromatogram

The tool is opened using the command "Tools -> Chromatogram" from the Main Window. The function is available if LC-MS data are processed. Use F1 to open this help.

Example Chromatogram

Toolbar

Export Image (CTRL + E)

Export the image into a PDF, PS (Windows only), PNG or SVG file. PS files in Linux can be generated outside of the application, e.g., using the pdf2ps tool.

Close (Esc)

Close window.

Zoom In (CTRL +)

Zoom in.

Zoom Out (CTRL -)

Zoom out.

Zoom Reset (CTRL + R)

Reset zoom.

Mouse Selection Tool (CTRL + T)

If the button is enabled, the mouse can be used to select a range retention time (or IDs) to be shown.

• Left mouse button - press and hold the button to draw a rectangle determining the minimum and maximum values of retention time (IDs); release the button to apply the changes.
• Right mouse button - press the button during the drawing of a rectangle to cancel the drawing.
• Middle mouse button - reset the range of retention time (IDs).
• Mouse wheel up - zoom the interval in.
• Mouse wheel down - zoom the interval out.

If the button is disabled, the mouse can be used to move the interval of retention time (IDs) to the left or right. Press and hold the left mouse button to shift the chromatogram.

Retention Time (CTRL + M)

View retention time or IDs of spectra.

Absolute Intensity (CTRL + I)

View absolute/relative intensities.

Profile Chromatogram (CTRL + P)

View profile chromatogram. The shape of peak in profile mode is estimated from peaks in line mode. The following functions are available to estimate the shape - Gaussian, ex-Gaussian (tailing), and ex-Gaussian (fronting).

Hide TIC (CTRL + Shift + T)

Hide/show total ion current chromatogram (TIC).

Hide Matched Peaks (CTRL + Shift + E)

Hide/show extracted ion chromatogram (EIC). EIC is calculated from the ions which are currently listed in Summary Table of Matched Peaks.

Hide Labels (CTRL + L)

The labels of chromatographic peaks are hidden if the button is checked.

HTML Documentation (F1)

Show HTML documentation.

Time

• First field - a minimum value of retention time to be shown.
• Second field - a maximum value of retention time to be shown.
• "Set Time" button (or Enter key if the field is focused) - apply the changes.
• "Reset Time" button - reset the range of retention time.

Spectrum ID

• First field - a minimum value of ID to be shown.
• Second field - a maximum value of ID to be shown.
• "Set ID" button (or Enter key if the field is focused) - apply the changes.
• "Reset ID" button - reset the range of IDs.