Laboratory Orientation

Laboratory combines three analytical tools for the characterization of molecular structure: mass spectrometry, nuclear magnetic resonance spectroscopy and microscopy.

MS group is focused mainly to proteomics and peptidomics fields, although some minor small molecule analysis has also been carried out from time-to-time. Peptide mapping, peptide sequencing and de novo sequencing approaches represent current routine protocols. For biomolecular separation prior to mass spectrometry 1D/2D chromatographic or gel methods are being employed. In February 2009 the complete Mass Imaging solution (MALDI, DESI) was installed on FTICR. Basic research in DAPCI, DAPPI and micro-chip fields is ongoing.

From January 2009 NMR operates a four channel 600 MHz instrument with a cryo-probe. This spectrometer is dedicated to protein analysis. In parallel, a dual channel 400 MHz instrument was installed and is dedicated to analysis of mostly low molecular weight natural compounds, their semisynthetic derivatives or biotransformation products.

Microscopy group runs multiple electron as well as optical microscopy equipment.



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