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Our laboratory combines three analytical tools for the characterization of molecular structure: mass spectrometry, nuclear magnetic resonance spectroscopy and electron microscopy. The instrumentation is spread in buildings A, C, U and L on campus. In addition to own scientific research, the contractual work is provided for academic and industrial partners (reQuest application).
Mass spectrometry group is focused on microbial metabolomics and multimodal imaging. Invasive pulmonary aspergillosis and Pseudomonas infections are studied in animal models by MALDI-FTICR imaging, scanning electron and optical microscopies. New diagnostic procedures are applied in human and veterinary medical mycology. Nuclear magnetic resonance group operates a four channel 700 MHz instrument with a cryo-probe dedicated to protein analysis. Analogous 600 MHz NMR instrument running in HPLC-NMR configuration is used in metabolomics projects (murine lipoatrophy and diabetes mellitus). It also can be on-line linked to a Q-TOF mass spectrometer. Microscopy group runs multiple electron as well as optical microscopy equipment. Scanning electron microscope FEI NanoSEM 450 is equipped with CBS detector for high resolution imaging of non-conductive samples and EDAX Octane Plus detector for EDS analysis and elemental mapping. Image data fusion is facilitated by own cross-platform tool called CycloBranch. In accord with other laboratory groups extensive service measurements are being carried out for the whole biological campus in Krč as documented by considerable number of collaborations and joint publications. The effective instrument sharing with Palacky University in Olomouc, Charles University in Prague, Purkinje University in Ústí nad Labem and the Institute of Chemical Technology in Prague is enabled through our part or full time scientists. Structural biology approaches are provided by our partner Laboratory of Structural Biology based in Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec.
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